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superscript iv one-step rt-pcr kit  (Thermo Fisher)


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    Thermo Fisher superscript iv one-step rt-pcr kit
    Superscript Iv One Step Rt Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iv+one-step+pcr+kit/superscript+iv+one+step+rt+pcr+kit/pmc12130083-65-12-17
    Average 90 stars, based on 1 article reviews
    superscript iv one-step rt-pcr kit - by Bioz Stars, 2026-10
    90/100 stars

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    One Step RT-PCR:

    Article Title: Molecular epidemiology of peste des petits ruminants virus emergence in critically endangered Mongolian saiga antelope and other wild ungulates
    Article Snippet: .. One-step RT-PCR was performed on 2 μl total RNA using the SuperScript IV One-Step PCR kit (ThermoFisher, Catalog No. 12594025) either to amplify nucleotides 1232–1583 of the PPRV N gene using published primers NP3 and NP4 ( ) or to amplify the full-length H gene (with primers 5ʹ-CTCCACGCTCCACCACAC-3ʹ and 5ʹ-CTCGGTGGCGACTCAAGG-3ʹ) or the full-length F gene (with primers 5ʹ- GCTATGCGGCCGCACCATGACGCGGGTCGCAATYTT-3ʹ and 5ʹ-GGTGAGGATCCCTACAGTGATCTTACGTACGAC-3ʹ). .. Total RNA from tissues was converted to complementary DNA with SuperScript IV and sequencing libraries prepared with the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1024).

    Article Title: Molecular epidemiology of peste des petits ruminants virus emergence in critically endangered Mongolian saiga antelope and other wild ungulates
    Article Snippet: .. One-step RT-PCR was performed on 2ul total RNA using the SuperScript IV One-Step PCR kit (ThermoFisher, Catalog No. 12594025) either to amplify nucleotides 1232-1583 of the PPRV N gene using published primers NP3 and NP4 (Couacy-Hymann et al 2002) or to amplify the full length H gene (with primers 5’-CTCCACGCTCCACCACAC-3’ and 5’-CTCGGTGGCGACTCAAGG-3’) or the full length F gene (with primers 5’-GCTATGCGGCCGCACCATGACGCGGGTCGCAATYTT-3’ and 5’-GGTGAGGATCCCTACAGTGATCTTACGTACGAC-3’). .. Total RNA from tissues was converted to cDNA with SuperScript IV and sequencing libraries prepared with the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1024).

    Polymerase Chain Reaction:

    Article Title: Molecular epidemiology of peste des petits ruminants virus emergence in critically endangered Mongolian saiga antelope and other wild ungulates
    Article Snippet: .. One-step RT-PCR was performed on 2 μl total RNA using the SuperScript IV One-Step PCR kit (ThermoFisher, Catalog No. 12594025) either to amplify nucleotides 1232–1583 of the PPRV N gene using published primers NP3 and NP4 ( ) or to amplify the full-length H gene (with primers 5ʹ-CTCCACGCTCCACCACAC-3ʹ and 5ʹ-CTCGGTGGCGACTCAAGG-3ʹ) or the full-length F gene (with primers 5ʹ- GCTATGCGGCCGCACCATGACGCGGGTCGCAATYTT-3ʹ and 5ʹ-GGTGAGGATCCCTACAGTGATCTTACGTACGAC-3ʹ). .. Total RNA from tissues was converted to complementary DNA with SuperScript IV and sequencing libraries prepared with the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1024).

    Article Title: Molecular epidemiology of peste des petits ruminants virus emergence in critically endangered Mongolian saiga antelope and other wild ungulates
    Article Snippet: .. One-step RT-PCR was performed on 2ul total RNA using the SuperScript IV One-Step PCR kit (ThermoFisher, Catalog No. 12594025) either to amplify nucleotides 1232-1583 of the PPRV N gene using published primers NP3 and NP4 (Couacy-Hymann et al 2002) or to amplify the full length H gene (with primers 5’-CTCCACGCTCCACCACAC-3’ and 5’-CTCGGTGGCGACTCAAGG-3’) or the full length F gene (with primers 5’-GCTATGCGGCCGCACCATGACGCGGGTCGCAATYTT-3’ and 5’-GGTGAGGATCCCTACAGTGATCTTACGTACGAC-3’). .. Total RNA from tissues was converted to cDNA with SuperScript IV and sequencing libraries prepared with the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1024).

    Article Title: Novel protocatechuic acid encapsulated bovine serum albumin functionalized folic acid nanoparticles for targeted therapy in urethane-induced lung cancer model
    Article Snippet: RNA extraction from mice lungs was done by Direct-zol RNA Miniprep Plus (Cat# R2072, ZYMO RESEARCH CORP. USA) and then the quality of RNA was assessed by Beckman dual spectrophotometer (USA). .. SuperScript IV One-Step PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA USA) was utilized for reverse transcription of extracted 2 μg RNA to produce cDNA followed by PCR in one step. ..

    Reverse Transcription:

    Article Title: Novel protocatechuic acid encapsulated bovine serum albumin functionalized folic acid nanoparticles for targeted therapy in urethane-induced lung cancer model
    Article Snippet: RNA extraction from mice lungs was done by Direct-zol RNA Miniprep Plus (Cat# R2072, ZYMO RESEARCH CORP. USA) and then the quality of RNA was assessed by Beckman dual spectrophotometer (USA). .. SuperScript IV One-Step PCR kit (Cat# 12594100, Thermo Fisher Scientific, Waltham, MA USA) was utilized for reverse transcription of extracted 2 μg RNA to produce cDNA followed by PCR in one step. ..



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